Circulation Research СHDAC3ػ
ǰ
ȫֲѾ “”ѧҽѧԺDr. Deqiang LiоŶ20227Circulation Research־ϽΪ“Epicardial HDAC3 promotes myocardial growth through a novel microRNA pathway”ѪоչĤHDAC3ͨmiR-322/miR-503̼Fgf9Igf2ļ ΪȱݵIJļṩµսԺͿ
侰
HDACѰȥø3һHDACijԱ ԴѰβлȥ ִͨﳡѧ߲ȫóHDAC3ɵ¼Դȱݺ̥ ļԵHDAC3ȱʧȴж̬ͱӰ ǻڵЧעHDAC3ڷǼϸҼĹЧܶļҪоּ̽Ѱ HDAC3ĤеԼFgf9Igf2ıܵHDACsmiRsĵ
ͼ. The schematics of the working model
Ҫ
1еĤHdac3ıļҪ
2HDAC3ĤϸֽǨǺҪ
3miR-322miR-503Fgf9Igf2ת¼
4HDAC3ͨmiR-322miR-503ıFgf9Igf2ת¼
о
01
Figure 1. Epicardial deletion of Hdac3 resulted in hypoplasia of ventricular compact wall.
02
Figure 2. Hdac3 deletion resulted in downregulation of Fgf9 and Igf2.
03
оͼȷFgf9Igf2̭Ƿļϸֳ̭йϸEV Hdac3óKOҺFgf9Igf2ϸ崦óͷE13.5ɢԭ̥ļϸEV KO崦óͷpH3ļϸٷֱȺļϸͲļϸֳźͨ·ERKữļKOFgf9Igf2ļϸֳȱݲʹp-FGFR1/p-IGFR1Чע ĤϸFgf9Igf2ṩļϸֳҪ
Figure 3. Supplementation of Fgf9 or Igf2 rescues CM proliferation defects.
04
Figure 4. HDAC3 induces the expression of Fgf9 and Igf2 dependent on its deacetylase activity.
05
FgfsIgfsıɱmiRs о߶ómiRͨģϵλɸѡmiR-322miR-503ģﴦóͷFgf9Igf2ıͨWestern blotһ֤miR-322 mimics/miR-503 mimicsóͷFgf9Igf2ʹmiR-322miR-503ģﴦóͷE13.5ļϸ pH3ļϸİٷֱ עmiR-322miR-503ļϸֳHDAC3ȥøԱRGFP966ʱ miR-322miR-503óͬϵE13.5 ekoҲӲ쵽miR-322miR-503ϵ֤ʵmiR-322miR-503ܹFgf9Igf2ļϸֳ
Figure 5.miR-322 and miR-503 repress the expression of Fgf9 and Igf2 and CM proliferation.
06
Figure 6. Knockdown of miR-322 or miR-503 restores the expression of Fgf9 and Igf2.
07
Figure 7. HDAC3 represses miR-322/miR-503 promoter activity.
Ѫܼлģʵ8Żݣսʵң
UGж긻ĶʵѪܶģӹʵ ߾ʵĻ벡ͬʱй˾ļƽ̨ƽ̨ϸƽ̨רҵʵƽ̨ ΪṩѪܶģӵԤ⡢ϸЧѧоһվʽѪоЧϵͳ 漰Ѫǰоֶ֧ӴעUGںʶʵЧ UGڴĵ
