NatureʹΪʲôȥ
ἰʩЧһ衣˵ʱԽһʩƯŮӵг֮ò֮òһʩʹʮܡһֻؿ˿ʩȴֱһˡʩһжʩòһʩؿüͷӦЧȴԽò
ص㽲ʹʹǻܵΣԴ̼ʱһֲĸܡ붯ʹշΪ1ӱΪϥַ䣻2¡˻ԼʹеʩơȻѡԵʹΪ·δ֪
20181213Nature־˹ѧҽѧԺQiufu MaڵҪ[1]Ƿ豳ټ1ԪͨͶ벢ʹշΪʹķӱΪо״ηʹΪ·Ƕʹ֪
Qiufu Ma
о֤ʵټ1ͣTAC1ԪʹΪ[2]߶ԴԪϸоǽTAC1-CreСtdTomatoӽTAC1-tdTomato ԪNK1Rڹ꣨ͼ1a-bNK1RǼ1ͶԪķӱǡ
ΪоTAC1ԪͶ䵽Щ߽TAC1-CreСCDX2-FlpoСӽԱǼβTAC1ԪTAC1ԪҪͶ䵽ֺˡԺˡڲԼԺҲͶ䵽ԺеطӱΪCGRPԪ[3]TAC1ǼβԪͶ䵽ڲԼԺ˸ͼ1c-h
ͼ1 TAC1ԪͶ䵽ڲԼԺ
Ϊо豳TAC1ԪĹЧ߽TAC1-CreСLBX1-FlpoСLBX1Ϊ豳Ƿӱ[4]ӽͨCre/Flpo˫ԱɱTAC1LBX1Ԫͼ2aǷɱTAC1LBX1ԪСԻе̼̼ȴ̼ķӱΪСͼ2b-fɱTAC1LBX1ԪС־Ӱ¶ѡҲκӰ죨ͼ2g-h
TAC1LBX1Ԫе¶ȡշʹķӱΪ
ͼ2 TAC1LBX1ԪʹķӱΪ
ЧʶTAC1LBX1ԪʹķӱΪôȺԪǷʹշΪԼǷλӱΪ ߾ʹо
ͬҪɱTAC1LBX1ԪСڳʱȰ塢ĩʹ̼צˡƤгΪʽЩʹշΪͼ3a-fڲ˵Ļ֢״ͬ[5]ɱTAC1LBX1ԪƤгִʹշλӱΪͼ3g
߾TAC1LBX1ԪͶһоTac1-CreС༹עAAV-ChR2ԺϷֲˡ⼤-ԺͶշλӱΪͼ3hעTAC1LBX1ԪͨͶʹΪ
ͼ3 TAC1LBX1ԪʹշΪ
Ϊоˮƽʽѧ빦ЧɢǷͬڳԾѡַǹʹTRPV1MRGPRD[6]оͼ4aǷɱMRGPRDԪСĻеʹֵΪɱTRPV1ԪСеʹʹֵȹΪͣͼ4b-cЩЧעTRPV1ԪMRGPRDԪʹΪоҪԡ
ͼ4 ʹΪTRPV1MRGPRDԪ
ʹٴһ֢״зԷӦԼ¡ΪȻڵʹΪ·δ֪ƪϵʾ١ŴѧΪѧȶҪ췢豳TAC1ԪΪʩչҪɱTAC1Ԫ̭ΪӰ췴ӱΪ豳TAC1Ͷ价·շʹΪоչʹΪ·ͼ5Ϊٴʹؼṩ֧֣
ͼ5 ʹΪ·ʾͼ
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1.Huang, T., et al., Identifying the pathways required for coping behaviours associated with sustained pain. Nature, 2018. 2.Gutierrez-Mecinas, M., et al., Preprotachykinin A is expressed by a distinct population of excitatory neurons in the mouse superficial spinal dorsal horn including cells that respond to noxious and pruritic stimuli. Pain, 2017. 158(3): p. 440-456. 3.Han, S., et al., Elucidating an Affective Pain Circuit that Creates a Threat Memory. Cell, 2015. 162(2): p. 363-374. 4.Duan, B., et al., Identification of spinal circuits transmitting and gating mechanical pain. Cell, 2014. 159(6): p. 1417-1432. 5.Young, R.F., et al., Gamma Knife thalamotomy for the treatment of persistent pain. Stereotact Funct Neurosurg, 1995. 64 Suppl 1: p. 172-81. 6.Cavanaugh, D.J., et al., Distinct subsets of unmyelinated primary sensory fibers mediate behavioral responses to noxious thermal and mechanical stimuli. Proc Natl Acad Sci U S A, 2009. 106(22): p. 9075-80.
